As the sixteen castaways arrived deep in the Amazon jungle, Jeff shocked the castaways when he divided the castaways into a tribe of men, Tambaqui, and a tribe of women, Jaburu. He gave the castaways their buffs and maps to their respective camps. As Tambaqui arrived at their camp, they had a quick meeting and questioned the women's survival skills. When Jaburu arrived at their camp, Christy shocked her tribemates by announcing that she's deaf. At the immunity challenge, Tambaqui took an early lead, however Jaburu pulled ahead when Daniel and Ryan had trouble with the balance beam. Immunity was won by Jaburu, sending the men to Tribal Council.